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blasticidin  (Thermo Fisher)


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    Structured Review

    Thermo Fisher blasticidin
    Blasticidin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hepes/Blasticidin+S+hydrochloride%2C+98%2B%25%2C+in+1M+HEPES+buffer+soln/pm42259351-86-15-21
    Average 97 stars, based on 1 article reviews
    blasticidin - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Isolation:

    Article Title: Exercise training-induced extracellular miR-136-3p modulates glucose uptake and myogenesis through targeting of NRDC in human skeletal muscle
    Article Snippet: Human embryonic kidney (HEK293) cells were obtained from American Type Culture Collection (ATCC) and cultured in high-glucose (4.5 g/L) Dulbecco's Modified Eagle Medium (DMEM; Thermo Fisher Scientific, Waltham, MA, USA), supplemented with 10% (vol/vol) FBS. .. Human islets were obtained from the University of Uppsala islet isolation core and were cultured in CMRL 1066 media (Thermo Fisher Scientific) with 1% GLUTAMAX (Thermo Fisher Scientific), 1% HEPES (Thermo Fisher Scientific), 5 mM sodium pyruvate (Thermo Fisher Scientific), 1% penicillin–streptomycin (Gibco, Carlsbad, CA, USA) and 12 μM nicotinamide (Merck, Darmstadt, Germany). ..

    Cell Culture:

    Article Title: Exercise training-induced extracellular miR-136-3p modulates glucose uptake and myogenesis through targeting of NRDC in human skeletal muscle
    Article Snippet: Human embryonic kidney (HEK293) cells were obtained from American Type Culture Collection (ATCC) and cultured in high-glucose (4.5 g/L) Dulbecco's Modified Eagle Medium (DMEM; Thermo Fisher Scientific, Waltham, MA, USA), supplemented with 10% (vol/vol) FBS. .. Human islets were obtained from the University of Uppsala islet isolation core and were cultured in CMRL 1066 media (Thermo Fisher Scientific) with 1% GLUTAMAX (Thermo Fisher Scientific), 1% HEPES (Thermo Fisher Scientific), 5 mM sodium pyruvate (Thermo Fisher Scientific), 1% penicillin–streptomycin (Gibco, Carlsbad, CA, USA) and 12 μM nicotinamide (Merck, Darmstadt, Germany). ..

    Article Title: Identification of long non-coding RNAs involved in leukemogenesis and venetoclax response in acute myeloid leukemia through functional CRISPR-dCas9 interference screens
    Article Snippet: .. AML cell lines HL60, NB4, U937, IMS-M2, F36P and THP1 were cultured in RPMI-1640 with GlutaMAX and HEPES (Gibco) supplemented with 10% heat-inactivated fetal bovine serum (FBS, Thermo Scientific), while MOLM-13 and Kasumi1 cells were cultured in medium supplemented with 20% FBS. .. KG1 were cultured in IMDM medium with GlutaMAXTM (Gibco) supplemented with 20% FBS and OCI-AML2 and OCI-AML3 were cultured in α-MEM (Gibco) supplemented with 20% FBS.

    Article Title: Metabolic determinants of cancer immunotherapy outcomes identified by plasma profiling.
    Article Snippet: Flat-bottom 96-well plates were coated with anti-CD3 antibody (OKT3, Invitrogen, 14-0037-82) at two suboptimal concentrations (0.05 μg ml−1 and 0.2 μg ml−1). .. Cells were cultured in complete RPMI 1640/GlutaMAX medium (GIBCO, 61870-010) supplemented with 10% heat-inactivated human AB serum (Institut Jacques Boy, 201021334), 1% penicillin–streptomycin (GIBCO, 15140-122), 1% HEPES (GIBCO, 15630-056), 1% sodium pyruvate (GIBCO, 11360-039) and 1% polymyxin B (final concentration 10 μg ml−1; Merck, 81271-10ML). .. T cells were seeded at 100,000 cells per well onto the anti-CD3-coated plates and cultured in the presence of increasing concentrations of L-histidine (Thermo Scientific, A10413.22) (0, 0.1, 0.3, 1, 3, 10 and 30 mM) for 48 h at 37 °C and 5% CO2.

    Article Title: A molecularly defined polymeric platform for environmentally responsive activation of STING to enhance cancer immunotherapy.
    Article Snippet: The stimulator of interferon genes (STING) pathway is a promising immuno-oncology target.. Despite their potential, STING agonists have yielded underwhelming results in clinical trials due to pharmacological barriers that limit their safety and efficacy.. Herein, we describe the design and pre-clinical evaluation of a polymeric dimeric amidobenzimidazole (diABZI) STING agonist delivery platform for cancer immunotherapy.

    Article Title: Optimized intracellular flow cytometry panel enables CD4 and CD8 T cell cytokine profiling in Syrian hamsters.
    Article Snippet: The Syrian golden hamster has emerged as a valuable animal model in challenge experiments and vaccine efficacy studies, particularly for infectious diseases that are poorly represented in mice.. In recent years, research has demonstrated that hamsters can better recapitulate certain human infectious diseases compared to mice.. Syrian hamsters are used in studies with Yellow fever virus, Helicobacter spp., Hantavirus pulmonary syndrome, Japanese encephalitis, Nipah virus, Adenovirus, Influenza A/B, and SARS-CoV-2, among others.

    Concentration Assay:

    Article Title: Metabolic determinants of cancer immunotherapy outcomes identified by plasma profiling.
    Article Snippet: Flat-bottom 96-well plates were coated with anti-CD3 antibody (OKT3, Invitrogen, 14-0037-82) at two suboptimal concentrations (0.05 μg ml−1 and 0.2 μg ml−1). .. Cells were cultured in complete RPMI 1640/GlutaMAX medium (GIBCO, 61870-010) supplemented with 10% heat-inactivated human AB serum (Institut Jacques Boy, 201021334), 1% penicillin–streptomycin (GIBCO, 15140-122), 1% HEPES (GIBCO, 15630-056), 1% sodium pyruvate (GIBCO, 11360-039) and 1% polymyxin B (final concentration 10 μg ml−1; Merck, 81271-10ML). .. T cells were seeded at 100,000 cells per well onto the anti-CD3-coated plates and cultured in the presence of increasing concentrations of L-histidine (Thermo Scientific, A10413.22) (0, 0.1, 0.3, 1, 3, 10 and 30 mM) for 48 h at 37 °C and 5% CO2.

    Modification:

    Article Title: A molecularly defined polymeric platform for environmentally responsive activation of STING to enhance cancer immunotherapy.
    Article Snippet: The stimulator of interferon genes (STING) pathway is a promising immuno-oncology target.. Despite their potential, STING agonists have yielded underwhelming results in clinical trials due to pharmacological barriers that limit their safety and efficacy.. Herein, we describe the design and pre-clinical evaluation of a polymeric dimeric amidobenzimidazole (diABZI) STING agonist delivery platform for cancer immunotherapy.

    Passaging:

    Article Title: Integrated mesenchymal and extracellular cues drive bioengineered liver tissue formation and function
    Article Snippet: .. Advanced DMEM/F12 (Gibco) supplemented with 1% (v/v) penicillin-streptomycin (Gibco), 1% (v/v) GlutaMax (Gibco), 10 mM HEPES (Gibco), was used as the basal medium to make organoid initiation medium, expansion medium (EM), differentiation medium (DM), and to wash organoids during passaging or sample collection. .. Other major materials used for organoid isolation and maintenance are as follows: type II collagenase (Gibco), dispase (Gibco), MatrigelTM (Corning), and non-attaching 24-well plates (M9312, Greiner, Merck).

    Suspension:

    Article Title: Optimized intracellular flow cytometry panel enables CD4 and CD8 T cell cytokine profiling in Syrian hamsters.
    Article Snippet: The Syrian golden hamster has emerged as a valuable animal model in challenge experiments and vaccine efficacy studies, particularly for infectious diseases that are poorly represented in mice.. In recent years, research has demonstrated that hamsters can better recapitulate certain human infectious diseases compared to mice.. Syrian hamsters are used in studies with Yellow fever virus, Helicobacter spp., Hantavirus pulmonary syndrome, Japanese encephalitis, Nipah virus, Adenovirus, Influenza A/B, and SARS-CoV-2, among others.



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